Changes of Beclin-1 and ULK1 in retina of mice model in oxygen-inducedretinopathy

Jie Wang; Ergang Du; FeiFei Li; Yunliang Zheng

doi:10.1016/j.aopr.2022.100065

您当前的位置：

首页 >

文章列表页 >

Changes of Beclin-1 and ULK1 in retina of mice model in oxygen-inducedretinopathy

更新时间：2023-11-15

- Changes of Beclin-1 and ULK1 in retina of mice model in oxygen-inducedretinopathy
- AOPR Vol. 2, Issue 3, Pages: 100065(2022)
- 作者机构：
  
  1. ,Hangzhou,China
  2. ,Hangzhou,China
  3. ,Hangzhou,China
- 作者简介：
- 基金信息：
- DOI：10.1016/j.aopr.2022.100065
  CLC：
扫描看全文
Jie Wang, Ergang Du, FeiFei Li, et al. Changes of Beclin-1 and ULK1 in retina of mice model in oxygen-inducedretinopathy. [J]. AOPR 2(3):100065(2022)
DOI：

Jie Wang, Ergang Du, FeiFei Li, et al. Changes of Beclin-1 and ULK1 in retina of mice model in oxygen-inducedretinopathy. [J]. AOPR 2(3):100065(2022) DOI： 10.1016/j.aopr.2022.100065.

摘要

Purpose,To observe the expression differences and potential effects of autophagy-related Beclin1 (mammalian Atg6) and Uncoordinated-51 like kinase 1 (ULK1) in the oxygen-induced retinopathy (OIR) model.,Materials and methods,Thirty-three C57BL/6 mice in OIR model group were exposed to 75 ± 0.5% oxygen from postnatal day-of-life 7 (P7) to P12, and were then brought into normal room environment (relative hypoxia stage) and raised to P17. Thirty-three control mice were kept in a normal room environment. The expression of autophagy in the retina tissue was assessed by Western blot analysis. The thickness and ultrastructural of retina were observed by light microscopy and transmission electron microscope (TEM) on P17.,Results,In the hyperoxia stage (P8–P11), the expression of Beclin1, ULK1 and Autophagy 5 (Atg5) in retina showed no significant difference between the OIR model group and the control group. In the relatively hypoxia stage (P14 to P17), however, the protein level of Beclin1, ULK1, and Bcl-2-associated X protein (Bax) were upregulated in the retina of the OIR model group, whereas B-cell lymphoma 2 (Bcl-2) was downregulated. The autophagosomes in the photoreceptors of retina in the OIR mice were observed. The inner-segment/out-segment (IS/OS) layer in OIR model group was thinner than that the control group on P17.,Conclusions,The expression of Beclin-1 and ULK1 in retina has changed in the OIR model, and the change of Beclin-1 and ULK1 expression is related to the change of oxygen concentration.

Abstract

关键词

Keywords

Retinopathy of prematurityAutophagyRetinaBeclin1Uncoordinated-51 like kinase 1 (ULK1)

references

1 A. Hellström, L.E. Smith, O. DammannRetinopathy of prematurity Lancet, 382 (9902) (2013), pp. 1445-1457, 10.1016/S0140-6736(13)60178-6

2 S.M. Tan, N. Stefanovic, G. Tan, et al.Lack of the antioxidant glutathione peroxidase-1 (GPx1) exacerbates retinopathy of prematurity in mice Invest Ophthalmol Vis Sci, 54 (1) (2013), pp. 555-562, 10.1167/iovs.12-10685

3 D. Clark, K. MandalTreatment of retinopathy of prematurity Early Hum Dev, 84 (2) (2008), pp. 95-99, 10.1016/j.earlhumdev.2007.11.007

4 H. Åkerblom, S. Andreasson, E. Larsson, et al.Photoreceptor function in school-aged children is affected by preterm birth Transl Vis Sci Technol, 3 (6) (2014), p. 7, 10.1167/tvst.3.6.7

5 R.M. Hansen, A. Moskowitz, J.D. Akula, et al.The neural retina in retinopathy of prematurity Prog Retin Eye Res, 56 (2017), pp. 32-57, 10.1016/j.preteyeres.2016.09.004

6 J. Wang, Y. Zheng, E. DuExpression and clinical significance of autophagy related genes in the retina of mice model of retinopathy of prematurity Journal of Zhejiang Chinese Medical University, 44 (5) (2020), pp. 426-429, 10.16466/j.issn1005-5509.2020.05.004

7 Z. Zhou, T.A. Doggett, A. Sene, et al.Autophagy supports survival and phototransduction protein levels in rod photoreceptors Cell Death Differ, 22 (3) (2015), pp. 488-498, 10.1038/cdd.2014.229

8 A. Kuma, N. MizushimaPhysiological role of autophagy as an intracellular recycling system: with an emphasis on nutrient metabolism Semin Cell Dev Biol, 21 (2010), pp. 683-690, 10.1016/j.semcdb.2010.03.002

9 C.G. Besirli, N.D. Chinskey, Q.D. Zheng, et al.Autophagy activation in the injured photoreceptor inhibits fas-mediated apoptosis Invest Ophthalmol Vis Sci, 52 (7) (2011), pp. 4193-4199, 10.1167/iovs.10-7090

10 E. Dehdashtian, S. Mehrzadi, B. Yousefi, et al.Diabetic retinopathy pathogenesis and the ameliorating effects of melatonin; involvement of autophagy, inflammation and oxidative stress Life Sci, 193 (2018), pp. 20-33, 10.1016/j.lfs.2017.12.001

11 L.E. Smith, E. Wesolowski, A. McLellan, et al.Oxygen-induced retinopathy in the mouse Invest Ophthalmol Vis Sci, 35 (1) (1994), pp. 101-111, 10.1007/BF00917835

12 E.Y. ChanRegulation and function of uncoordinated-51 like kinase proteins Antioxidants Redox Signal, 17 (5) (2012), pp. 775-785, 10.1089/ars.2011.4396

13 E. Itakura, N. MizushimaCharacterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins Autophagy, 6 (2010), pp. 764-776, 10.4161/auto.6.6.12709

14 S. Pattingre, A. Tassa, X. Qu, et al.Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy Cell, 122 (6) (2005), pp. 927-939, 10.1016/j.cell.2005.07.002

15 S. Mukhopadhyay, P.K. Panda, N. Sinha, et al.Autophagy and apoptosis: where do they meet? Apoptosis, 19 (4) (2014), pp. 555-566, 10.1007/s10495-014-0967-2

16 L. Ouyang, Z. Shi, S. Zhao, et al.Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis Cell Prolif, 45 (6) (2012), pp. 487-498, 10.1111/j.1365-2184.2012.00845.x

17 F. Sennlaub, Y. Courtois, O. GoureauInducible nitric oxide synthase mediates retinal apoptosis in ischemic proliferative retinopathy J Neurosci, 22 (10) (2002), pp. 3987-3993, 10.1523/JNEUROSCI.22-10-03987.2002

18 S.P. Narayanan, J. Suwanpradid, A. Saul, et al.Arginase 2 deletion reduces neuro-glial injury and improves retinal function in a model of retinopathy of prematurity PLoS One, 6 (7) (2011), Article e22460, 10.1371/journal.pone.0022460

19 P. Boya, L. Esteban-Martínez, A. Serrano-Puebla, et al.Autophagy in the eye: development, degeneration, and aging Prog Retin Eye Res, 55 (2016), pp. 206-245, 10.1016/j.preteyeres.2016.08.001

20 P. Chai, H. Ni, H. Zhang, X. FanThe evolving functions of autophagy in ocular Health: a double-edged sword Int J Biol Sci, 12 (11) (2016), pp. 1332-1340, 10.7150/ijbs.16245

21 M. Cammalleri, F. Locri, E. Catalani, et al.The beta adrenergic receptor blocker propranolol counteracts retinal dysfunction in a mouse model of oxygen induced retinopathy: restoring the balance between apoptosis and autophagy Front Cell Neurosci, 11 (2017), p. 395, 10.3389/fncel.2017.00395

22 G. Casini, M. Dal Monte, I. Fornaciari, et al.The β-adrenergic system as a possible new target for pharmacologic treatment of neovascular retinal diseases Prog Retin Eye Res, 42 (2014), pp. 103-129, 10.1016/j.preteyeres.2014.06.001

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Research progress of iron metabolism in retinal diseases

Retinal safety and toxicity study of artesunate in vitro and in vivo

Role of Rapamycin and 3-MA in oxidative damage of HLECs caused by two doses of UVB radiation

Related Author

No data

Related Institution

Department of Ophthalmology, The First Affiliated Hospital of Harbin Medical University

Department of Ophthalmology, Shenzhen Third People's Hospital

The First Affiliated Hospital of Harbin Medical University

Department of Ophthalmology, Li Ka Shing Faculty of Medicine, The University of Hong Kong

State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong

⁰